![]() ![]() IEF standards: use 5 µl per mini gel for Coomassie staining or 0.5 µl for silver staining to yield 50 or 500 applications, respectively. The plugs can be used on vertical 2D gels with no reference well. Precision Plus Protein unstained standard plugs allow easy, quick, and clean loading of molecular weight standards on any gel. * 2-D standards: use 2.5 µl per mini gel for Coomassie staining (200 applications) or 0.5–2.5 µl for silver staining (up to 1,000 applications) use 1.0–5.0 µl for full-length gels (16–20 cm) to yield 100 or up to 500 applications, respectively. Precision Plus Protein Standards for protein electrophoresis contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD. (1610374) and TidyBlot Western Blot Detection Reagent-HRP (STAR209) were purchased from BioRad (Marne-la-Coquette (14). For IÎ✫α protein detection, the blotting membrane was blocked with 5 BSA (Roth 8076.2) in a tris. 2-D SDS-PAGE protein standards provide calibrated references for protein pI and molecular weight in the second dimension. A protein ladder was purchased from (Bio-Rad 1610374). IEF and 2D protein standards are a mixture of native proteins with isoelectric points (pI) ranging from 4.45 to 9.6, providing reproducible pI calibration in native PAGE or agarose IEF gels. Choose prestained, tagged, or fluorescent standards. (Note: Actual weights may vary lot-specific MWs are included with each vial.) Recommended for use with Bio-Rad's TGX Stain-Free Precast Gels and TGX Stain-Free FastCast Acrylamide. IEF and 2D Electrophoresis Protein Standards Select from an extensive range of protein ladders for SDS PAGE, western blotting, IEF and 2D-SDS PAGE. Precision Plus Protein Unstained Recombinant Protein Standards are Strep -tagged, enabling immunodetection and molecular weight determination on western blots. ![]()
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